氮钾浓度对烤烟苗期生长特性的影响

探究氮钾浓度对烟苗生理生化特性的影响,为烟叶苗期氮钾营养平衡提供参考依据.采用水培法,利用基础Hoagland营养液,设置5个不同氮钾浓度处理,分析处理间烟苗的生理生化指标差异.结果表明,氮浓度对烟苗干物质、叶绿素的积累有显著影响,在相同钾浓度下,随着氮浓度的增加,烟苗干物质、叶绿素含量、根系活力显著增加.不同氮水平下,低钾处理间的根系MDA含量、叶片GS活性差异显著.K+对烟苗叶绿素的合成、根系活力、GS活性有一定的促进作用.高氮水平下,高浓度K+会使根系活力、叶片可溶性蛋白含量下降.     

Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions

Synthetic hexaploid wheat(SHW), possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties. We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-L1) and a common wheat line, under normal(NC) and polyethylene glycol-simulated drought stress conditions(DC). We mapped quantitative trait loci(QTLs) for root traits using an enriched high-density genetic map containing 120 370 single nucleotide polymorphisms(SNPs), 733 diversity arrays technology markers(DArT) and 119 simple sequence repeats(SSRs). With four replicates per treatment, we identified 19 QTLs for root traits under NC and DC, and 12 of them could be consistently detected with three or four replicates. Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7% of the phenotypic variation respectively, and six novel QTLs for root fresh weight, the ratio of root water loss, total root surface area, number of root tips, and number of root forks under DC explained 8.5–14% of the phenotypic variation. Here seven of eight novel QTLs could be consistently detected with more than three replicates. Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.     

绿盲蝽象在马莲小枣上的危害和防治

绿盲蝽象对枣强县马莲小枣的危害逐年加重。本文介绍绿盲蝽象在马莲小枣上的危害特点、发生规律与生活习性,提出综合防治措施。     

水稻畦式好气栽培法试验

以传统栽培为对照,研究畦式好气栽培对水稻根系、群体消长、产量构成因素及产量的影响。结果表明,畦式好气栽培显著提高了整个生育期水稻根系的生物量,与传统栽培相比,增幅达5.46%－11.18%;显著增加了抽穗后10cm以下土层的根系比例和根系活力,提高了根冠比,促进了水稻分蘖的发生,显著提高了水稻有效穗数、结实率和产量,每公顷可比传统栽培增产稻谷637.5kg,增产9.48%。     

鲜食玉米设施栽培

针对近年来甜糯等鲜食玉米在我市种植面积迅速扩大的现状，本文根据田间试验点总结出鲜食玉米超早熟设施栽培技术。旨在为广大农民实际生产应用提供参考依据。     

牧草中的水溶性碳水化合物

牧草中有适量的水溶性碳水化合物对家畜及牧草都有十分重要的意义。本文从牧草中水溶性碳水化合物的种类、影响因素、贮存过程中的损失和对家畜的营养价值等方面加以综述。     

一个与稻瘟病菌无毒基因AVR-Pik~m连锁的SCAR标记的分离

 本研究将以前在稻瘟病菌菌株S1522获得的与决定对水稻品种梅雨明无毒性的基因(AVR-Pik^m)相连锁的1个RAPD标记OPO12₁₀₀₀进行了克隆和鉴定。核苷酸序列测定与分析结果表明:OPO12₁₀₀₀的大小为946个碱基,不含有与已报道的稻瘟病菌Mg-SINE、Fosburry、Magyy、Grasshopper、Pot2以及Pot3等同源的重复序列。根据OPO12₁₀₀₀的核苷酸序列,设计了1对24个核苷酸的特异引物,对无毒表型亲本S1522和毒性表型亲本S159、无毒表型群体基因池、毒性表型基因池以及有性杂交后代108个菌株进行了PCR扩增,所有无毒表型的菌株均能特异性地扩增出1条与OPO12₁₀₀₀大小相同的DNA条带,而毒性表型的菌株除5个重组个体外,均不能扩增出这条特异带。此结果表明,与稻瘟病菌无毒基因AVR-Pik^m连锁的RAPD标记OPO12₁₀₀₀被成功地转化为SCAR标记,为进一步通过染色体步移克隆该无毒基因奠定了基础。     

体尺细分法在竞赛用伊犁马选择上的应用研究

本文采用体尺细分法 ,测定了乌鲁木齐马术俱乐部 2 4匹伊犁马 1 9个细分体尺及 1 0 0 0m速度成绩。分析细分体尺与速度的相关性 ,结果为 :颈长、肩长、肱骨长、股骨长、胫骨长、肩倾角、股骨倾角、髋骨倾角与速度呈显著 (P <0 0 5)或极显著 (P <0 0 1 )相关     

The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.     

贺兰山西坡不同海拔梯度草地土壤氮特征

以阿拉善左旗境内贺兰山中段(西坡)及山前地带不同海拔下的草地为研究对象,分析不同海拔梯度下草地土壤全氮的分布特征及其与气候因子、植被特征和土壤特性的关系.结果表明,土壤全氮含量随海拔的降低而逐渐降低,在典型草原中沿土壤垂直剖面依次降低,在高寒草甸、荒漠化草原和草原化荒漠0～20与20～40 cm土层中差异不显著,但高于40～60 cm土层.在海拔1 360～1 380 m草原化荒漠的5个样地上,由于放牧利用程度和植被类型的不同,0～20 cm土层土壤氮的含量变幅在0.022 8%～0.034 7%.0～20,20～40和40～60 cm土层土壤全氮含量与年降水量、植被盖度、草地生产力、土壤含水量、有机碳含量和<0.05 mm颗粒含量呈极显著正相关,与年均温、土壤pH值呈极显著负相关(P<0.001).偏相关分析显示,影响0～20 cm土层全氮(TN)含量最主要的因素是植被盖度(C)和年降水量(AP),相关方程是TN%=0.002 040 C 0.000 585 AP-0.076 800;而影响20～40 cm土层全氮含量的最主要因素是草地生产力(B),与土壤全氮回归方程为TN%=0.001 6 B 0.024 7.而各环境因素中影响土壤全氮含量最主要的因素,0～20 cm土层为土壤有机碳和年降水量,20～40 cm土层主要受土壤有机碳的影响.